产品名称：丝/苏氨酸蛋白质激酶II α抗体

一抗操作步骤：

洗涤：‌洗涤的目的是为了洗去一抗的非特异性结合，‌洗涤的效果直接影响结果背景的深浅。‌建议使用PBST或TBST作为洗涤液，‌其中的Tween20有助于去除非特异性的结合，‌减少背景。‌洗涤的次数和时间根据具体实验来确定，‌建议清洗3-5次，‌每次5分钟。

丝/苏氨酸蛋白质激酶II α抗体

英文名称：CK II alpha

中文名称：丝/苏氨酸蛋白质激酶II α抗体

别    名；STK; Casein kinase II Alpha; CKII alpha; Serine/Threonine Protein Kinase II Alpha; CKⅡalpha; Casein kinase 2 alpha 1 polypeptide; Casein kinase II alpha 1 subunit; Casein kinase II alpha chain; Casein kinase II alpha subunit; Casein kinase II subunit alpha; Casein kinase II alpha 1; CK II alpha; CKIIalpha; CSK21_HUMAN; CK II; CK2 alpha; CK2 catalytic subunit alpha; CK2A1; CKII; CSNK2A1.

研究领域；细胞生物  信号转导  细胞凋亡  激酶和磷酸酶

抗体来源；Rabbit

克隆类型；Polyclonal

交叉反应；Human,  (predicted: Mouse, Rat, Dog, Cow, )

产品应用；WB=1:500-2000 ELISA=1:5000-10000

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量；45kDa

细胞定位；细胞核

性    状；Liquid

浓    度；1mg/ml

免 疫 原；KLH conjugated synthetic peptide derived from human CK II alpha: 201-300/391

亚    型；IgG

纯化方法；affinity purified by Protein A

保存条件；Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍：

Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV. Phosphorylates PML at 'Ser-565' and primes it for ubiquitin-mediated degradati

抗体孵育注意事项：‌使用干净的孵育盒子，‌避免油脂等杂物沾到膜上造成非特异信号。‌如果一次性需要检测多个蛋白，‌可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜

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