商品属性：
产品名称
低分子量蛋白7抗体
货号
BJ010437
英文名称
PSMB8
浓 度
1mg/ml
研究领域
细胞生物 细胞周期蛋白 细胞分化
细胞定位
细胞核 细胞浆
抗体来源
Rabbit
性 状
Liquid
克隆类型
Polyclonal
亚 型
IgG
理论分子量
23kDa
用途
仅供科研研究实验
商品介绍：
别 名
Proteasome 20S LMP7; D6S216; D6S216E; Large
multifunctional peptidase 7; Large multifunctional protease 7; LMP 7; LMP-7;
LMP7; Low molecular mass protein 7; Low molecular weight protein 7; Macropain
subunit C13; MGC1491; Multicatalytic endopeptidase complex subunit C13;
Protease component C13; Proteasome (prosome macropain) subunit beta type 8;
Proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional
peptidase 7); Proteasome beta 8 subunit; Proteasome catalytic subunit 3i;
Proteasome component C13; Proteasome related gene 7; Proteasome subunit beta
5i; Proteasome subunit beta type 8; Proteasome subunit beta type; Proteasome
subunit beta type-8; Proteasome subunit beta-5i; Proteasome subunit Y2; PSB8_HUMAN;
PSMB 8; PSMB5i; Really interesting new gene 10 protein; RING 10; RING10; Y2;
ALDD; D6S216E; JMP; NKJO; PSMB5i; RING10.
交叉反应；Human, (predicted: Mouse, Rat, Dog, Cow, Horse,
Rabbit, Sheep, )
产品应用；WB=1:500-2000
ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in
other applications.
optimal
dilutions/concentrations should be determined by the end user.
免 疫 原；KLH conjugated synthetic
peptide derived from human Proteasome 20S LMP7: 101-200/276
亚 型；IgG
纯化方法；affinity purified by Protein A
缓 冲 液；0.01M TBS(pH7.4) with 1% BSA,
0.03% Proclin300 and 50% Glycerol.
注意事项；This product as supplied is
intended for research use only, not for use in human, therapeutic or diagnostic
applications.
低分子量蛋白7抗体产品介绍：
The proteasome is
a multicatalytic proteinase complex with a highly ordered ring-shaped 20S
core structure. The core structure is composed of 4 rings of 28 non-identical
subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed
of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells
at a high concentration and cleave peptides in an ATP/ubiquitin-dependent
process in a non-lysosomal pathway. An essential function of a modified
proteasome, the immunoproteasome, is the processing of class I MHC peptides.
This gene encodes a member of the proteasome B-type family, also known as the
T1B family, that is a 20S core beta subunit. This gene is located in the
class II region of the MHC (major histocompatibility complex). Expression of
this gene is induced by gamma interferon and this gene product replaces
catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome.
Proteolytic processing is required to generate a mature subunit. Two
alternative transcripts encoding two isoforms have been identified; both
isoforms are processed to yield the same mature subunit. [provided by RefSeq,
Jul 2008].
The proteasome is
a multicatalytic proteinase complex which is characterized by its ability to
cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving
group at neutral or slightly basic pH. The proteasome has an ATP-dependent
proteolytic activity. This subunit is involved in antigen processing to
generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases
the capacity of the immunoproteasome to cleave model peptides after
hydrophobic and basic residues. Acts as a major component of interferon
gamma-induced sensitivity. Plays a key role in apoptosis via the degradation
of the apoptotic inhibitor MCL1. May be involved in the inflammatory response
pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1)
results in immunoproteasome deficiency. Required for the differentiation of
preadipocytes into adipocytes.
一抗是一种球蛋白，能特异性结合特定蛋白或重点研究的其他生物分子，目的是对其进行纯化、检测和定量。使用小鼠、大鼠、兔子、山羊和其他动物作为宿主，可获得多克隆或单克隆抗体。
一抗的生产和类型有多种形式，从抗血清粗品到抗原纯化制剂不等。
我们常说的抗体指的是第抗体。市售的一抗有多种不同的生产方式，这些不同导致抗体各具特性，适用于不同的应用。了解每种抗体的特性对选择合适的抗体至关重要。
单克隆抗体和多克隆抗体都是我们实验中常用的抗体产品。他们在识别的抗原表位、生产和应用等方面有一些区别。
✓多克隆抗体（polyclonal antibody, pAb）：用一种包含多种抗原决定簇的抗原动物，可刺激机体多个 B 细胞克隆产生针对多种抗原表位的抗体。
✓单克隆抗体（monoclonal antibodies, mAb）：由单一 B 细胞克隆产生的识别一种抗原表位的抗体。
双特异性抗体实验流程 ：
1. 目标选择：
确定目标：确定需要靶向的相关蛋白或细胞表面受体。
确定机制：确定的作用机制，例如靶向细胞表面受体促进细胞凋亡，或者阻断病原体的进入等。
2. 抗体设计：
设计抗体结构：设计能够同时结合两个不同抗原的抗体结构。
确定结构特征：确定双特异性抗体的结构特征，例如连接方式、抗原结合位点等。
3. 抗体工程：
克隆基因：通过分子生物学技术克隆出编码双特异性抗体的基因序列。
重组表达：将基因插入适当的表达系统（通常是哺乳动物细胞或大肠杆菌）进行表达和生产。
4. 抗体表征：
亲和度分析：使用生物物理学技术（如表面等离子共振、生物层析等）评估抗体与抗原的亲和度。
功能性分析：评估抗体的生物学活性，例如在细胞模型中测试其对靶标的影响。
5. 生产和纯化：
大规模生产：将优选的双特异性抗体基因转染至合适的细胞系，进行大规模生产。
纯化：使用柱层析、亲和层析等技术对抗体进行纯化。
6. 功能验证：
体外实验：在细胞系或体外模型中评估双特异性抗体的活性和毒性。
动物模型：在动物模型中评估双特异性抗体的药效学和性。
公司正在出售的产品：
INO80D蛋白抗体
6磷酸果糖激酶1抗体 Anti-PFK1
INO80D
类固醇硫酸酯酶抗体 Anti-SSDD/Steroid sulfatase
3号染色体开放阅读框67抗体
Donkey Anti-Goat
IgG H&L / PE
C3orf67
FRZB蛋白抗体
跨膜γ羧基酸蛋白4抗体
PE标记的驴抗羊IgG H&L
细胞诱导型巨噬细胞一氧化氮合成酶（iNOS /NOS2/mNOS）活性荧光定量检测试剂盒
FRZB/FRP-3
小鼠中性粒细胞趋化因子-1（CINC-1）elisa检测试剂盒
犬2,3-二磷酸甘油酸(2,3-DPG)elisa分析检测试剂盒
大鼠催产素(OT)elisa检测试剂盒
小鼠胱天蛋白酶5(CASP-5)elisa分析检测试剂盒
人补体蛋白3(C3)elisa检测试剂盒免费代测
CASP5ELISAkit
PRRG4
3-DPG ELISA Kit
球蛋白A诱导源蛋白抗体
猴slit源物2(果蝇)(SLIT2)elisa分析检测试剂盒
IGIP
Monkey slit
homolog 2(Drosophila)(SLIT2)ELISA kit
纺锤体和着丝粒相关蛋白1抗体 Anti-SKA1
人多聚腺苷二磷酸核糖聚合酶(PARP)elisa分析检测试剂盒
PDZ结构域蛋白GOPC抗体 Anti-PIST/GOPC
PARPELISAKit
低分子量蛋白7抗体细胞表面趋化因子受体4相关蛋白2抗体 CNOT2 研究领域；细胞生物 表观遗传学 VWCE抗体 VWCE 及病毒 ATP酶钙离子转运蛋白PMCA3抗体 PMCA3 研究领域； 细胞生物 神经生物学 信号转导 转运蛋白 链激酶抗体 Streptokinase 研究领域；心血管 细胞生物 微生物学 及病毒