商品属性：
产品名称
MEK1/MAPKK1抗体(N-端)
货号
BJ011259
英文名称
MEK1
浓 度
1mg/ml
研究领域
细胞生物 神经生物学 信号转导 细胞凋亡 激酶和磷酸酶
细胞定位
细胞核 细胞浆
抗体来源
Rabbit
性 状
Liquid
克隆类型
Polyclonal
亚 型
IgG
理论分子量
43kDa
用途
仅供科研研究实验
商品介绍：
别 名
mitogen activated protein kinase kinase 1; Dual
specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1;
MAPKK 1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK1; Map2k1; MAPKK1; Mek1;
MEKK1; Prkmk1; MP2K1_HUMAN.
交叉反应；Human, (predicted: Mouse, Rat, Chicken, Dog, Pig,
Rabbit, )
产品应用；ELISA=1:5000-10000
IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in
other applications.
optimal
dilutions/concentrations should be determined by the end user.
免 疫 原；KLH conjugated synthetic
peptide derived from human MEK1: 2-150/393
亚 型；IgG
纯化方法；affinity purified by Protein A
缓 冲 液；0.01M TBS(pH7.4) with 1% BSA,
0.03% Proclin300 and 50% Glycerol.
注意事项；This product as supplied is
intended for research use only, not for use in human, therapeutic or diagnostic
applications.
MEK1/MAPKK1抗体(N-端)产品介绍：
Dual specificity
protein kinase which acts as an essential component of the MAP kinase signal
transduction pathway. Binding of extracellular ligands such as growth
factors, cytokines and hormones to their cell-surface receptors activates RAS
and this initiates RAF1 activation. RAF1 then further activates the
dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both
MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade,
and catalyze the concomitant phosphorylation of a threonine and a tyrosine
residue in a Thr-Glu-Tyr sequence located in the extracellular
signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their
activation and further transduction of the signal within the MAPK/ERK
cascade. Depending on the cellular context, this pathway mediates diverse
biological functions such as cell growth, adhesion, survival and
differentiation, predominantly through the regulation of transcription,
metabolism and cytoskeletal rearrangements.
Dual specificity
protein kinase which acts as an essential component of the MAP kinase signal
transduction pathway. Binding of extracellular ligands such as growth
factors, cytokines and hormones to their cell-surface receptors activates RAS
and this initiates RAF1 activation. RAF1 then further activates the
dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both
MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade,
and catalyze the concomitant phosphorylation of a threonine and a tyrosine
residue in a Thr-Glu-Tyr sequence located in the extracellular
signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their
activation and further transduction of the signal within the MAPK/ERK
cascade. Depending on the cellular context, this pathway mediates diverse
biological functions such as cell growth, adhesion, survival and
differentiation, predominantly through the regulation of transcription,
metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK
cascade is peroxisome proliferator-activated receptor gamma (PPARG), a
nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has
been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also
involved in the regulation of endosomal dynamics, including lysosome
processing and endosome cycling through the perinuclear recycling compartment
(PNRC), as well as in the fragmentation of the Golgi apparatus during
mitosis.
一抗是一种球蛋白，能特异性结合特定蛋白或重点研究的其他生物分子，目的是对其进行纯化、检测和定量。使用小鼠、大鼠、兔子、山羊和其他动物作为宿主，可获得多克隆或单克隆抗体。
一抗的生产和类型有多种形式，从抗血清粗品到抗原纯化制剂不等。
我们常说的抗体指的是第抗体。市售的一抗有多种不同的生产方式，这些不同导致抗体各具特性，适用于不同的应用。了解每种抗体的特性对选择合适的抗体至关重要。
单克隆抗体和多克隆抗体都是我们实验中常用的抗体产品。他们在识别的抗原表位、生产和应用等方面有一些区别。
✓多克隆抗体（polyclonal antibody, pAb）：用一种包含多种抗原决定簇的抗原动物，可刺激机体多个 B 细胞克隆产生针对多种抗原表位的抗体。
✓单克隆抗体（monoclonal antibodies, mAb）：由单一 B 细胞克隆产生的识别一种抗原表位的抗体。
双特异性抗体实验流程 ：
1. 目标选择：
确定目标：确定需要靶向的相关蛋白或细胞表面受体。
确定机制：确定的作用机制，例如靶向细胞表面受体促进细胞凋亡，或者阻断病原体的进入等。
2. 抗体设计：
设计抗体结构：设计能够同时结合两个不同抗原的抗体结构。
确定结构特征：确定双特异性抗体的结构特征，例如连接方式、抗原结合位点等。
3. 抗体工程：
克隆基因：通过分子生物学技术克隆出编码双特异性抗体的基因序列。
重组表达：将基因插入适当的表达系统（通常是哺乳动物细胞或大肠杆菌）进行表达和生产。
4. 抗体表征：
亲和度分析：使用生物物理学技术（如表面等离子共振、生物层析等）评估抗体与抗原的亲和度。
功能性分析：评估抗体的生物学活性，例如在细胞模型中测试其对靶标的影响。
5. 生产和纯化：
大规模生产：将优选的双特异性抗体基因转染至合适的细胞系，进行大规模生产。
纯化：使用柱层析、亲和层析等技术对抗体进行纯化。
6. 功能验证：
体外实验：在细胞系或体外模型中评估双特异性抗体的活性和毒性。
动物模型：在动物模型中评估双特异性抗体的药效学和性。
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